Revital Goldshmid – Biotechnology, Technion Haifa
A Mesenchymal Stem Cell “star” … MSCs culture in a 3D microgel,

Yonatan Lati – Department of Nano Science and Nano Technology Technion Haifa
Confocal image of cell clusters in 3D PEG-Fibrinogen microcarriers, after suspension culture in a bioreactor. Morphology of HeLa cells in PF (7.5 mg/ml) microcarrier suspended in a bioreactor was examined by confocal microscopy (scale bar = 50μm). Microcarriers were taken from the bioreactors and fixed for actin/nucleus staining. Staining performed with Fluorescent phalloidin-FITC (green) marking actin filaments and DAPI for nucleus staining (blue). The represented image shows cell proliferation and growth of clusters after 21 days in incubation.

Yonatan Lati – Department of Nano Science and Nano Technology Technion Haifa.
Confocal image of cell cluster in 3D PEG-fibrinogen microcarriers after suspension culture in a bioreactor. Morphology of MCF-7 cells in PF (7.5 mg/ml) microcarrier suspended in a bioreactor was examined by confocal microscopy (scale bar = 50μm). Microcarriers were taken from the bioreactors and fixed for actin/nucleus staining. Staining performed with Fluorescent phalloidin (red) for actin and SYTOX® Green dye for nucleuses. The represented photo image cell proliferation and growth of cluster after 21 days in incubation.

Eran Gross, Dana Benes Dahan and Wayne D. Kaplan, Department of Materials Science and Engineering, Technion, Haifa


SEM micrograph of the fracture surface of a silicon carbide (6H – SiC) sintered with spark plasma sintering (SPS) at 2100°C for 30min with no sintering additives, showing a porous microstructure, as a part of a research on the sintering physics of silicon carbide ceramics. The image was taken using a FEG high resolution SEM with an in-lens secondary electrons detector.

Luba Kolik and Dganit Danino, Department of Biotechnology and Food Engineering, Technion, Haifa


A light microscope image of giant vesicles composed of PCDA and geraniol (surfactant/cosurfactant) mixture formed after a dilution of a vesicle gel in water. The vesicles were “bouncing” on a drop of water trapped under the coverslip.

Noa Ofer and Kinneret Keren, Faculty of Physics, Technion, Haifa


Differential ends labeling of actin filaments in MDA-231 human breast cancer cells. An overlay image of cells stained for actin filaments (AF488-phalloidin; green), free barbed ends (AF546-actin; red) and free pointed ends (AF647-actin; blue). Scale bar: 20 microns.

Erez Cohen, Haim Weissman and Boris Rybtchinski, Department of Organic Chemistry, Weizmann Institute of Science, Rehovot


This cryo-TEM micrograph (false colors) demonstrates a columnar assembly of star shaped self-assembled nanotubes made from an amphiphilic perylene diimide derivative in pure water. The diameter of the stars is 5.1 nm.

Margarita Kovtanyuk, Department of Structural Biology, Weizmann Institute of Science, Rehovot


SEM micrograph of coccolite remains on surface of the gizzard plate of cephalaspidean gastropod mollusk Philine aperta. As the vast majority of Cephalaspidea lack teeth, their digestive tract possesses a muscular oesophagal crop, the gizzard, which incorporates calcareous masticatory plates acting as a millstone which crushes injected food items. This SE image was produced by an InLens-detector of FEG-SEM (LEO-Supra-55VP). Working conditions: EHT 3kV, WD 2mm, Aperture Size 30μm.

Netta Vidavsky, Department of Structural Biology, Weizmann Institute of Science, Rehovot


Cryo-SEM view of the embryonic sea urchin spicule formation process within the cellular environment.
Inset: back scattered electrons image, emphasizing the mineralized spicule.

Ruth Moshe and Wayne D. Kaplan, Department of Materials Science & Engineering, Technion, Haifa


This image shows ready to press (RTP) alumina powder. The sample was prepared to examine the effect of initial powder microstructure on the final sintered sample. The image was acquired using a high resolution scanning electron microscope in secondary electron mode.

Roy Shiloh, Department of Physical Electronics, Fleischmann Faculty of Engineering, Tel Aviv University, Tel Aviv


A computer-generated hologram, measured with a FEI F20 TEM in the diffraction plane in LAD mode (910m) at 200kV. The scale bar was calculated as θ/ where θ= px 256 / 910, px 25 m , the pixel size 256 the length of the bar in pixels. The holographic mask, placed in the specimen holder, was fabricated using a focused ion-beam miller by drilling a two-dimensional array of ~60nm dips with a 100nm periodicity. The depth of each dip was carefully calculated using an iterative Fourier-transform algorithm
It is possible, under the appropriate conditions, to create such holograms in the diffraction plane of a STEM, for example, thus yielding a nanometer probe with a desired pattern

Palle von Huth and Ziva Lapidot, University of Haifa and Israel Oceanographic and Limnological Research, Haifa


A view down the mouth opening of a sea coral polyp. Tentacles covered with sting cells surround the opening. Some of the stinging arrows are visible as thin threads. Several tentacles also contain spherical cells of sea algae that live in symbiosis with the coral. Imaged with the new Nikon A1R confocal laser microscope at the University of Haifa, Faculty of Natural Sciences.

Elisaveta Kossoy, Haim Weissman and Boris Rybtchinski, Department of Organic Chemistry, Weizmann Institute of Science, Rehovot


This cryo-TEM micrograph (false colors) demonstrates a self-assembled 2.0 nm molecular wire shaped as a spiral 48 nm in diameter. The assembly is held together by cobalt-water bridges among the amphiphilic perylene diimide cobalt complexes in 19:1 water:THF. The high contrast regions of the molecular wire fine structure (0.7 nm each) is due to highly aromatic moieties (ca. 1.2 nm high) of the assembled molecules. Lower right: a satellite image from the Kalahari desert of spiral shaped geoglyphs made by an unknown ancient civilization in South Africa. Each spiral is tens to hundreds of meters wide.

Hadas Strenlicht and Wayne D. Kaplan, Department of Materials Science and Engineering, Technion, Haifa


TEM micrograph of magnesium oxide particles. Acquired in
the FEI Tecnai G2 T20

Anat Akiva, Department of Structural Biology, Weizmann Institute of Science, Rehovot


The zebrafish skeleton in a 30 days post fertilization larva.
The zebrafish bones start forming at the age of 5 days post fertilization with the first formation of the skull. The ossification will continue in a segmented fashion of the backbone from the distal to the proximal parts, whereas the tail will be the last to be formed. When the zebrafish reached the age of 30 days post fertilization it has already ossified all his bones.
The calcein is fluorescent marker which has high affinity to calcium ions. Immersing the living fish in calcein solution for several minutes, results in green fluorescent labeling of all the newly formed bones. Using this fluorescent indicator allows us to monitor in vivo the bone formation process.

Amir Gabay, Ruth Moshe and Wayne Kaplan, Department of Materials Science and Enginering, Technion, Haifa


This image shows a polished cross section of an alumina sample sintered at 1600°C in air displaying a speck of dust. The sample was examined for grain size analysis. The image was acquired using secondary electron microscope in secondary electron mode.

Alexander Rabkin, Ilse katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev


Copper sulfide nanoparticles synthesized by the single source, single solvent/surfactant method. The image was taken with a transmission electron microscope. The sample was prepared by dispersion in chloroform and drop-evaporation on a carbon coated grid.

Gal Mor Khalifa, Department of Structural Biology, Weizmann Institute of Science , Rehovot


Foraminifera are marine protozoans which construct an outer protective clacitic shell. The process by which the foraminifera cell incorporates calcium ions form the sea water and deposit them as a calcite mineral in its shell wall constitute large part of the calcium carbonate formation in the ocean. We study the process of the foraminifera shell formation by labeling calcium in the sea water with the fluorescent calcium indicator, clacein, and following its incorporation and distribution in the foraminifera cytoplasm and finally its incorporation into the shell wall. This is a freeze fractured, calcein labeled foraminifera cell observed in confocal fluorescent microscope under cryo conditions. Labeled with calcein we see the outline of the shell wall and the distribution of calcium in the cytoplasm. The red signal comes from auto fluorescence of algal symbionts living inside the cytoplasm of the foraminifera.

Hadar (Bratt) Nahor and Wayne D. Kaplan, Department of Materials Science and Engineering, Technion, Haifa


A secondary electron HRSEM micrograph of a single-crystal Ni particle on a (111) YSZ substrate. The Ni particle is oriented with the (111) plane parallel to the substrate surface and is covered by several grains of a different phase containing mostly Si and O. Although covered by another phase, the characteristic crystal shape of Ni can be easily recognized.
In this work, agglomeration of a Cr-doped Ni film (“solid state dewetting”) on YSZ substrates was carried out to form equilibrated Ni(Cr)-YSZ interfaces. Due to Si contamination in the furnace, the agglomerated Ni particles were covered by an un-expected polycrystalline layer.

Giuliano Bellapadrone and Michael Elbaum, Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot


A confocal fluorescence (left) and differencial interference contrast (right) image of fluorescent protein aggregates isolated from bacteria. The fusion of yellow fluorescent protein and ferritin protein produces molecules with high self aggregation tendency that once expressed at high yields and isolated from E. coli tend to assemble in large molecular structures. Scale bar in the images is 50 micrometers.

Galit Atiya and Wayne D. Kaplan, Department of Matetials Science and Engineering, Technion, Haifa


This image shows typical sunflower (Pt-silicide particle) which was formed during solid-state dewetting. The annealing of the sample was performed under flowing 99.9999% pure Ar+7%H2 at 1300˚C for 7 hours, with the presence of undesired Si contamination.
The image was acquired using high resolution scanning electron microscope using secondary in-lens detector.

Lior Embon, Yonathan Anahory, Yuri Myasoedov, Michael L. Rappaport, Martin E. Huber and Eli Zeldov, Department of Condensed Matter Physics, Weizmann Institute of Science, Rehovot


Magnetic vortices in a superconducting lead film as imaged by the scanning SQUID-on-tip microscope. All four images were taken at a temperature of 4.2 Kelvin and show the same area of 12 x 12 μm2 in which the film was shaped as an hour-glass. The images show the behavior of “vortex matter” at different densities. Electric current which is applied through the film exerts a force on these quantum entities and they start “flowing” across the film from the bottom upwards forming beautiful channels which resemble the flow of rivers or the branching of a tree.

Eyal Shimoni – Weizmann Institute of Science

Allon Weiner – Department of Materials and Interfaces, Weizmann Institute of Science
Plasmodium falciparum, the causative agent of Malaria. Segmented 3D data set acquired by serial surface view FIB/SEM.

Anna Fendyur
The Hebrew University of Jerusalem, ISRAEL
Network morphology and neuron-glia relationships of 20 days old hippocampal cells cultured on a cysteine terminated engulfment-promoting peptide (EPP). Confocal image of immunocytochemical staining of neurons with neuronal marker NF (green), of glial cells with GFAP marker (blue), counterstained with nuclear marker DAPI (red). Hippocampal cells grown on the EPP cluster to form large aggregates in which the glia cells form a sheet in contact with the substrate and the neurons grow on top. Cells in different aggregates interconnect by thick fascicles emanating from clusters and projecting to adjacent aggregates.

Anna Fendyur
The Hebrew University of Jerusalem, ISRAEL
Network morphology and neuron-glia relationships of 20 days old hippocampal cells cultured on poly-D-lysine laminin. Confocal image of immunocytochemical staining of neurons with neuronal marker NF (green), of glial cells with GFAP marker (blue), counterstained with nuclear marker DAPI (red).