PDB single

Record Info

 

Participant Info

Participant details

instrumentation

Microscopes, Sample preparation, Analysis tools, Special detectors

Instrument Name
AFS / AFS2 freeze substitution device
Manufacturer
Leica Microsystems
Special Detectors
Instrument Photo
Instrument Description

Device for freeze substitution. AFS is an older device using a controller for temperature control. AFS2 is a newer generation, using PC controlled temp.

The device allows for the gentle dehydration of samples frozen by high pressure freezing. Dehydration is conducted usually in acetone containing chemical fixatives (OsO4, Glutaraldehyde etc.). Once dehydrated, samples can be warmed up and embedded in plastic resin and further processed  for observation in the TEM.

Lab Details

Details of the the labs and how to contact them

Lab name
Electron Microscopy Unit
Lab details
Centralized EM facility
Lab location
Old Wolfson Building / Department of Chemical Research Support

Contact details

Contact person
Eyal Shimoni
Contact email
shimoni.eyal (at) gmail.com
Contact phone
Contact mobile
Contact fax