Yonatan Lati – Department of Nano Science and Nano Technology Technion Haifa.
Confocal image of cell cluster in 3D PEG-fibrinogen microcarriers after suspension culture in a bioreactor. Morphology of MCF-7 cells in PF (7.5 mg/ml) microcarrier suspended in a bioreactor was examined by confocal microscopy (scale bar = 50μm). Microcarriers were taken from the bioreactors and fixed for actin/nucleus staining. Staining performed with Fluorescent phalloidin (red) for actin and SYTOX® Green dye for nucleuses. The represented photo image cell proliferation and growth of cluster after 21 days in incubation.
